ELECTROPHORESIS OF PROTEIN FRACTIONS FROM BOAR SEMINAL PLASMA, VESICULAR SECRETION AND EPIDIDYMAL PLASMA

Proteomics of boar seminal plasma

Epididymal secretion of CD52 as measured in human seminal plasma by a fluorescence immunoassay.

A major secretory protein of the human epididymis that is taken up by maturing spermatozoa is homologous to the leukocyte antigen CD52. The epididymis was shown to be the sole source of CD52 in seminal fluid, since CD52 could be detected in seminal plasma from sperm-containing ejaculates and not in ejaculates of vasectomized patients by Western blot analysis. The glycoprotein is not expressed i...

Boar sperm cryosurvival is better after exposure to seminal plasma from selected fractions than to those from entire ejaculate.

Boar bulk ejaculates are now being collected instead of usual sperm-rich fractions (SRF) for artificial insemination purpose. The present study evaluated the influence of holding boar sperm samples before freezing surrounded in their own seminal plasma (SP), from either fractions/portions or the entire ejaculate, on post-thawing sperm quality and functionality. Ejaculates collected as bulk (BE)...

A ram epididymal secretory protein shares common antigenic determinants with rat epididymal proteins and human seminal plasma proteins.

Mammalian spermatozoa leave the testis as immature cells unable to fertilize until they have traversed the epididymis. Sperm maturation is an orchestrated series of sequential events occurring at different levels of the epididymis : sperm-zona pellucida binding ability in the proximal epididymis, progressive motility and the ability to penetrate and fertilize the oocyte in the middle epididymis...

Protein analysis of boar seminal plasma proteins with protective effect during low-temperature storage of spermatozoa

The present study aimed to investigate the effect of different seminal plasma proteins (SPPs) on boar spermatozoa functional characteristics. We investigated the putative protective effect of SSPs on sperm cells motility and velocity, as well as on the integrity of the plasma membrane (PM) during low-temperature storage at 4 °C. SPPs fractions were obtained and purified by gel permeation chroma...